Designed whole-cell-catalysis-assisted synthesis of 9,11-secosterols

• Marek Kõllo,
• Marje Kasari,
• Villu Kasari,
• Tõnis Pehk,
• Ivar Järving,
• Margus Lopp,
• Arvi Jõers and
• Tõnis Kanger

Beilstein J. Org. Chem. 2021, 17, 581–588, doi:10.3762/bjoc.17.52

• toxic oxidants. Keywords: chemoenzymatic synthesis; cortisol; hydroxylation; secosterol; whole-cell catalysis; Introduction Developments in the chemistry of steroids have stimulated extensive research interest in the exploration of new synthetic methods since the 1960s. Advances in synthetic biology

Engineering Pichia pastoris for improved NADH regeneration: A novel chassis strain for whole-cell catalysis

• Martina Geier,
• Christoph Brandner,
• Gernot A. Strohmeier,
• Mélanie Hall,
• Franz S. Hartner and
• Anton Glieder

Beilstein J. Org. Chem. 2015, 11, 1741–1748, doi:10.3762/bjoc.11.190

• render the resulting process economical and sustainable. For this purpose, biotransformations based on whole cells represent an elegant strategy as the cell metabolism can be exploited for cofactor supply. We have generated a novel platform strain for whole-cell catalysis based on the methylotrophic

Towards practical biocatalytic Baeyer- Villiger reactions: applying a thermostable enzyme in the gram- scale synthesis of optically- active lactones in a two-liquid- phase system

• Frank Schulz,
• François Leca,
• Frank Hollmann and
• Manfred T. Reetz

Beilstein J. Org. Chem. 2005, 1, No. 10, doi:10.1186/1860-5397-1-10

• this oxidation are valuable intermediates in prostaglandin synthesis.[31][32][46] We first evaluated the stereoselectivity of the oxidation of 1 in standard whole-cell catalysis using WT-PAMO and the three mutant enzymes (P1-P3) recently reported by our group (Scheme 1).[45] For the WT-PAMO we found

• case of CHMO-catalyzed oxidation of the same substrate.[31] In order to overcome this inhibition we tested the use of a second liquid phase in whole-cell catalysis using a water-immiscible organic phase to serve as substrate reservoir and product sink. Full conversion could be obtained using

• allow more favorable partitioning of the reactants between the aqueous and organic phase. Such organic solvents, however, are not compatible with whole-cell catalysis.[47] We therefore evaluated an in vitro catalysis approach, initially using phenylacetone (4) as the substrate. Such catalysis using